IN VITRO PROPAGATION OF BARLERIA PRIONITIS LINN AND ITS ANTIBACTERIAL ACTIVITY

Abstract

Protocol for plant propagation through shoot tips and leaf were established for Vajradanti- Barleria prionitis L. (Acanthaceae). Vajradanti is a known medicinal plant and its value in medicine is known since ages. The tissue culture of vajradanti has not been established and therefore the work was undertaken to develop the protocol. Murashige and Skoog’s (MS) medium with following 2,4-D, Kinetin, BAP and IAA were tested for growth. In 0.0125mg/l concentration of 2,4-D and 0.0125mg/l concentration of Kinetin callus induction from leaves was the fastest i.e. in 3 days. In shoot culture the fastest callus induction was in 4 days with 2,4 D -.025mg/ml and Kinetin -.025mg/ml. Antibacterial activity was also measured and it was found that in ether extract callus gave most antibacterial activity.