Formulation, Optimization and Evaluation of Nanoparticulate In-Situ Gel of Anthocyanin from Bilberry for Ocular Inflammation

Abstract

A natural source of anthocyanins that is highly concentrated is the bilberry (Vaccinium myrtillus L.). Bilberries and other berry fruits are known for their numerous health advantages. These polyphenolic components are thought to be the primary bioactive responsible for the bilberry's blue/black hue and strong antioxidant content. Even yet, the main benefit of bilberry is often touted as improved eyesight. Most people are most familiar with bilberries because of their supposed benefits for eyesight, especially at night. According to 2-year research, consuming 120 mg of bilberry anthocyanins daily improved visual performance by around 30% in persons with glaucoma, a disorder that causes progressive blindness; in contrast, the placebo group had a worsening visual function. Ionotropic gelation was used to create nine distinct batches (F1–F9) of bilberry-loaded Nanoparticulate In-situ gel, which was then homogenized to minimize size. When encapsulation efficiency (EE%) of the resultant Nanoparticles was measured, it was found that formulation F3 (2 gm calcium chloride, 3 gm sodium alginate) had the greatest EE% of Bilberry loaded Nanoparticles, 92.33±0.38. An assessment of the glaucoma efficacy was conducted using a selected batch (F3) combined with chitosan, HPMC, and Pluronic F127 gel. The drug release of formulation F1-F3 was found to be 82.11, 88.12, 93.0 % respectively. The drug release formulation F3 being the maximum.