Gymnema sylvestre Leaf Hydroalcoholic Extract: Promising Source of Antioxidants with Remarkable Yield and Potent Scavenging Activity

Abstract

This research study aimed to compare the antioxidant activity and yield of different extracts of leaf of Gymnema sylvestre, including methanolic, hydroalcoholic, and aqueous extracts. In recent years, extensive research has been conducted to explore the antioxidant potential of Gymnema sylvestre extracts. However, a comprehensive comparison of these extracts in terms of their yield and antioxidant activity is lacking. Therefore, the objective of this study was to evaluate and compare the yield and antioxidant activity of different extracts of Gymnema sylvestre, specifically methanolic, hydroalcoholic, and aqueous extracts. The percentage yield of each extract was determined, providing valuable insights into the efficiency of the extraction methods. Furthermore, the antioxidant activity of the extracts was assessed by measuring their scavenging activity and determining the IC50 values. Methanolic, hydroalcoholic, and aqueous extracts of Gymnema sylvestre leaf were obtained using the Soxhlet extraction method after being finely pulverized. Within the limitations of this study, it was concluded that the hydroalcoholic extract of Gymnema sylvestre exhibited the highest percentage yield, with a recorded value of 10.27 percent. The IC50 values for the Gymnema extract and Ascorbic acid were determined to be 59.09 µg/ml and 44.90 µg/ml, respectively. These findings indicate the potential antioxidant activity of Gymnema sylvestre extracts, with the hydroalcoholic extract showing the most promising results in terms of both yield and scavenging activity. Further investigations are needed to explore the underlying mechanisms and assess the potential health benefits of these extracts in various applications.