FORMULATION AND DEVELOPMENT OF REPAGLINIDE MICROPARTICLES BY IONOTROPIC GELATION TECHNIQUE

Abstract

The present investigation involves formulation and evaluation of microparticles with repaglinide as model drug for prolongation of drug release time. An attempt was made to prepare microparticles of repaglinide by ionotropic gelation technique, with a view to deliver the drug at sustained or controlled manner in gastrointestinal tract and consequently into systemic circulation. The microparticles were formulated by calcium chloride cross-linking method using various concentration of Hydroxy Propyl Methyl Cellulose and Chitosan by dropping the Drug-Polymer solution along with sodium alginate in calcium chloride solution. The prepared microparticles were evaluated for Flow behavior, Compatibility study, Drug Entrapment Efficiency, In-vitro Dissolution, Scanning Electron Microscopy and Sieving method. Among the seven formulations prepared and evaluated F1 and F4 are found to show satisfactory results. The Prepared Microparticles shows entrapment efficiency of 78.62% to 91.25% Fourier transform Infrared spectroscopy confirmed the absence of any DrugPolymer interaction. In-vitro release studies carried out in 1.2 pH and 7.2 pH phosphate buffer solution shows 93.78% and 91.78% of drug was released from F1 and F4 respectively. The results of Flow behavior and Particle size analysis were found to be in the official limits. The In-vitro release studies shows that formulation F1, F2 and F3 releases 91.99 %, 81.66 % and 71.66 % respectively after 12 hours. Formulation F4, F5 and F6 shows release of 92.11%, 81.93% and 81.76% respectively. Formulation F7 which is a combination of hydroxy propyl methyl cellulose and Chitosan shows drug release of 71.88%.