DEVELOPMENT AND CHARACTERIZATION OF ROSIGLITAZONE LOADED PLGA NANOPARTICLES USING TWO STEP DESOLVATION METHOD

Abstract

Application of nanotechnology in drug delivery system has opened up new areas of research in sustained release of drugs. The nanoparticles have the advantages of reaching otherwise less accessible sites in the body by escaping phagocytosis and entering tiny capillaries. Sustained release of the drug from the nanoparticles could maintain the therapeutic concentration for long durations. Rosiglitazone loaded PLGA nanoparticles were prepared by two step desolvation method. The nanoparticles were characterized for various parameters. Drug encapsulation efficiency was found to be between 82-90%. In vitro release study was conducted across a spectrapore membrane (cut off 3500 Da) precluding PLGA. Nanoparticle formulation released the drug at a sustained rate for prolonged duration (80% drug released at the end of 32 h). The release pattern followed the Korsmeyer Peppas equation. PLGA nanoparticles also exhibited excellent redispersibility and minimal increase in particle size. The results indicated that two step desolvation method is well suited to prepare PLGA nanoparticles and the process variables of the procedure can be fine-tuned depending on the clinical applications.