INFLUENCE OF TDZ ON THE IN VITRO PROPAGATION OF VALERIANA JATAMANSI

Abstract

Valeriana Jatamansi is a critically endangered medicinal herb of the genus Valeriana .It is generally exploited for its roots and rhizomes and is useful as a sedative , antispasmodic , carminative, nervine and a stimulating agent .Valerian ,its main compound has been traditionally used in treatment of sleeping problems, obesity, epilepsy and snake poisoning .The aim of this study was to establish a practical methodology for rapid and large scale multiplication of Valeriana Jatamansi combining varying concentrations of growth regulators like THIDIAZURON (TDZ) and Kinetin. The results showed that using lower concentrations of THIDIAZURON (TDZ) and Kn (TDZ 1mg/l +Kn 0.5mg/l)after 9-10 days helped in induction of longer shoots while using higher concentrations (TDZ 5mg/l+ Kn 4 mg/l) within 5-6 days resulted in increase of leaf size and number of shoots.